Two-label peak-height encoded DNA sequencing by capillary gel electrophoresis: three examples
نویسندگان
چکیده
منابع مشابه
Two-label peak-height encoded DNA sequencing by capillary gel electrophoresis: three examples.
We report a modification to the peak-height encoded DNA sequencing technique of Tabor and Richardson. As in the original protocol, the sequencing reaction uses modified T7 polymerase with manganese rather than magnesium to produce very uniform incorporation of each dideoxynucleoside. To improve sequencing accuracy, two fluorescently labeled primers are employed in separate sequencing reactions....
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.18.4873